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Calculate peptide concentration from UV-Vis absorbance at 280nm using Beer-Lambert law (A = εlc).
Ideal range: 0.1 – 1.0 for accurate readings
Molar Concentration
335.57 µM
3.356e-4 M
Mass Concentration
476.3423 mg/mL
476342.28 µg/mL
Equation
c = A / (ε × l) = 0.5 / (1490 × 1)
c = 3.3557e-4 M
Based on Beer-Lambert law. Extinction coefficients assume native, non-denatured peptides. Accuracy depends on sample purity and proper blanking. For research purposes only.