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Enter peak areas from your HPLC chromatogram to calculate purity percentage, identify impurity concerns, and understand your results.
This tool is for educational purposes only. HPLC purity is one measure of peptide quality and does not replace full analytical characterization. Area-percent purity assumes equal UV response factors for all peaks, which is an approximation.
The integrated area of the main peptide peak from your chromatogram.
Helps classify impurity types based on relative retention.
The HPLC column used for separation.